Invitrogen™ GPR149 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532801

Catalog No. PIPA532801

Description

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Elephant (94%); Monkey, Marmoset (88%); Bat (81%). For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes.

Specifications

• Antigen: GPR149
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GPR149
• Gene Alias: G protein-coupled receptor 149; G protein-coupled receptor PGR10; GPR149; G-protein coupled receptor PGR10; IEDA; PGR10; Probable G-protein coupled receptor 149
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 344758
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin)
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: Q86SP6
• Gene Symbols: GPR149
• Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human GPR149.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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