Invitrogen™ GRAMD1A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA552950

Catalog No. PIPA552950

Description

Immunogen sequence: SSTGEEADLA ALLPDLSGRL LINSVFHVGA ERLQQMLFSD SPFLQGFLQQ CKFTDVTLSP WSGDSKCHQR RVLTYTIPIS NPLGPKSASV VETQTLFRRG PQAGGCVVDS EVLTQGIPYQ DYFYTAHRY Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.

Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER. Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol. In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain. At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER. May play a role in tumor progression. Plays a role in autophagy regulation and is required for biogenesis of the autophagosome (PubMed:31222192). This function in autophagy requires its cholesterol-transfer activity (PubMed:31222192). [UniProt].

Specifications

• Antigen: GRAMD1A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GRAMD1A
• Gene Alias: 1300003M23Rik; AI415002; AW547240; D7Bwg0611e; GRAM domain containing 1A; GRAM domain-containing protein 1A; GRAMD1A; KIAA1533; Protein Aster-A
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 57655
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q96CP6
• Gene Symbols: GRAMD1A
• Immunogen: Recombinant protein corresponding to Human GRAMD1A. Recombinant protein control fragment (Product #RP-90007).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG