Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. BDB743053

Granulocytes Mouse Anti-Rat, Brilliant Violet 421, Clone: RP-1, BD Optibuild™

Catalog No. BDB743053 Shop All BD Biosciences Products
Change view
Click to view available options
Quantity:
50 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
BDB743053 50 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. BDB743053 Supplier BD Biosciences Supplier No. 743053
Add to Cart
Add to Cart

Description

Mouse Monoclonal Antibody

The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells. It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments. Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb. Expression of the RP-1 antigen on rat peritoneal neutrophils is enhanced by stimulation with PMA or ConA. Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85 kDa in m.w. However, the RP-1 antigen has not been biochemically characterized.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Granulocytes
Classification Monoclonal
Clone RP-1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen WKA/Hok rat peritoneal neutrophils
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.