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Granulocytes Mouse Anti-Rat, Brilliant Violet 786, Clone: RP-1, BD Optibuild™

Catalog No. BDB743058 Shop All BD Biosciences Products
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Catalog No. BDB743058 Supplier BD Biosciences Supplier No. 743058
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Description

Mouse Monoclonal Antibody

The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells. It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments. Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb. Expression of the RP-1 antigen on rat peritoneal neutrophils is enhanced by stimulation with PMA or ConA. Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85 kDa in m.w. However, the RP-1 antigen has not been biochemically characterized.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Granulocytes
Classification Monoclonal
Clone RP-1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 786
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen WKA/Hok rat peritoneal neutrophils
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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