Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Granzyme B Rat anti-Mouse, Clone: 16G6, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 14882282

 View more versions of this product

Catalog No. 50-112-9084


Add to cart

Description

Description

Description: The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis. For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com. Applications Reported: This 16G6 antibody has been reported for use in western blotting, immunohistochemical staining, ELISA capture antibody, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: The unlabelled 16G6 antibody has been tested as the capture antibody in a sandwich ELISA for quantitation of mouse granzyme B protein levels, in combination with the biotinylated eBioLUEE (13-8821) antibody for detection and Recombinant mouse granzyme B as the standard. A suitable range of concentrations of this antibody for ELISA detection is 1-4 µg/mL. The 16G6 antibody has been tested for Western blotting; a recommended starting concentration is 1 µg/mL. The 16G6 antibody has been used for formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Specifications

Specifications

Granzyme B
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04187
GrB
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
ELISA, Immunohistochemistry (Paraffin), Western Blot
16G6
Unconjugated
Gzmb
Liquid
Gzmb
IgG2b, kappa
100 μg
4° C
Primary
14939
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.