Granzyme B Recombinant Rabbit Monoclonal Antibody (BLR022E), Bethyl Laboratories

Rabbit Recombit Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-157-1298	Bethyl Laboratories, Inc A700022T	10 μL
50-157-1297	Bethyl Laboratories, Inc A700022	100 μL

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommende

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Specifications

• Antigen: Granzyme B
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P10144
• Gene ID (Entrez): 3002
• Immunogen: Recombit protein representing Human Granzyme B
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: GZMB

• Clone: BLR022E
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
• Gene Alias: GrB
• Gene Symbols: GZMB
• Isotype: IgG
• Purification Method: Protein A
• Product Type: Antibody