Invitrogen™ GRK2 Monoclonal Antibody (3F8)

Catalog No.	Quantity
PIMA515840	100 μL

Catalog No. PIMA515840 Supplier Invitrogen™ Supplier No. MA515840

Description

Mouse Monoclonal Antibody

MA5-15840 targets GRK2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples. The MA5-15840 immunogen is purified recombinant fragment of human GRK2 expressed in E. Coli. . MA5-15840 detects GRK2 which has a predicted molecular weight of approximately 80kDa.

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

Specifications

• Antigen: GRK2
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3F8
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: ascites with 0.03% sodium azide
• Gene: GRK2
• Gene Accession No.: P25098, P26817, Q99MK8
• Gene Alias: ADRBK1; Adrbk-1; adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; BARK; BARK1; Bark-1; beta ARK; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; betaARK1; BETA-ARK1; beta-ARK-1; EC 2.7.1.126; G protein-coupled receptor kinase 2; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2; Grk2; GRK-2
• Gene Symbols: GRK2
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 110355, 156, 25238
• Target Species: Human, Mouse, Rat, Monkey
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1