GRK2 Polyclonal Antibody, Rockland™

Goat Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600101MH5

Catalog No. 50-199-1579

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human GRK2. A BLAST analysis of the sequence shows 100% reactivity to human, and 90% to rat, mouse, and golden hamster.

G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., ?2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs ,regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

Specifications

• Antigen: GRK2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GRK2
• Gene Alias: Adrbk1; Adrbk-1; adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; BARK; BARK1; Bark-1; beta ARK; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; betaARK1; BETA-ARK1; Beta-ARK-1; EC 2.7.1.126; G protein-coupled receptor kinase 2; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; GRK2; GRK-2
• Host Species: Goat
• Regulatory Status: RUO
• Gene ID (Entrez): 110355, 156
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Concentration: 0.89 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P25098, Q99MK8
• Gene Symbols: GRK2
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody