Invitrogen™ GRP94 Monoclonal Antibody (9G10), PerCP

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA545098

Catalog No. PIMA545098

Description

0.5 μg/mL of MA5-45098 was sufficient for detection of Grp94 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. Detects approximately 98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

Specifications

• Antigen: GRP94
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.2
• Gene Accession No.: O18750, P08110, P08113, P14625, P41148, Q29092, Q66HD0, Q95M18
• Gene Symbols: HSP90B1, hsp90b1.L
• Immunogen: Purified Grp94 isolated from chicken oviducts.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Sheep, Hamster, Pig, Horse, Chicken, Guinea Pig, Frog
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 9G10
• Conjugate: PerCP
• Gene: HSP90B1
• Gene Alias: 108K heat shock protein; 108K HSP; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250; 98 kDa protein kinase; ecgp; endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250; endoplasmin-like protein; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; glucose-regulated protein; glucose-regulated protein GRP94; glucose-regulated protein GRP94 precursor; GP96; gp96 homolog; gp96/GRP94; GRP94; GRP-94; heat shock 108 kDa protein; heat shock protein 108; heat shock protein 108.; heat shock protein 90 beta family member 1; heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90, beta, member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein 90kDa beta family member 1 L homeolog; heat shock protein 90kDa beta, member 1; heat shock protein gp96; HEL35; HEL-S-125m; HSP 108; hsp108; HSP90 beta; hsp90b1; hsp90b1 {ECO:0000250; hsp90b1.L; Polymorphic tumor rejection antigen 1; PPK 98; Ppk 98; a protein kinase; ppk98; stress-inducible tumor rejection antigen gp96; TA-3; Targ2; tra1; Tra-1; transferrin-binding protein; transforming growth factor alpha regulated gene 2; tumor rejection antigen; tumor rejection antigen (gp96) 1; tumor rejection antigen 1; Tumor rejection antigen gp96; UniProtKB:P08113}; XELAEV_18017384mg
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100009456, 100033933, 100723767, 100773331, 101111703, 22027, 282646, 362862, 374163, 397191, 399408, 404019, 7184
• Content And Storage: 4°C
• Form: Liquid