Invitrogen™ GSDMD Monoclonal Antibody (6D11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549242

Catalog No. PIMA549242

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human THP-1 whole cell, human SW620 whole cell, human Jurkat whole cell, human U87 whole cell, human PC-3 whole cell. IHC: human cervical cancern tissue, human cervical intraepithelial neoplasia tissue, human esophageal squamous carcinoma tissue. ICC/IF: PC-3 cell. Flow: Jurkat cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

Specifications

• Antigen: GSDMD
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P57764
• Gene Symbols: GSDMD
• Immunogen: E.coli-derived human GSDMD recombinant protein (Position: M1-H484).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 6D11
• Conjugate: Unconjugated
• Gene: GSDMD
• Gene Alias: 1810036L03Rik; AW558049; DF5L; Dfna5l; FKSG10; gasdermin D; gasdermin domain containing 1; gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; GSDMD; GSDMDC1; GSDMD-CT; GSDMD-NT; M2-4
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79792
• Content And Storage: -20°C
• Form: Lyophilized