Invitrogen™ GTF2B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528556

Catalog No. PIPA528556

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa nuclear extract, GTF2B-transfected 293T. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (95%), Xenopus laevis (96%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Specifications

• Antigen: GTF2B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GTF2B
• Gene Alias: general transcription factor 2B L homeolog; general transcription factor IIB; general transcription factor TFIIB; GTF2B; gtf2b.L; gtf2b-a; gtf2b-b; LOW QUALITY PROTEIN: transcription initiation factor IIB; MGC137096 protein; RNA polymerase II alpha initiation factor; RNA polymerase II transcription factor IIB; RP11-82K18.1; S300-II; TF2B; tfiib; Transcription initiation factor IIB; Transcription initiation factor IIB-like protein; wu:fc44c11; XELAEV_18023356mg; XLTFIIB; zgc:66258
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 229906, 2959
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P62915, Q00403
• Gene Symbols: GTF2B
• Immunogen: Recombinant fragment corresponding to a region within amino acids 22 and 316 of Human TFIIB.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG