Invitrogen™ GTF3C3 Polyclonal Antibody

Catalog No.	Quantity
PIPA5120636	100 μL

Catalog No. PIPA5120636 Supplier Invitrogen™ Supplier No. PA5120636

Description

Rabbit Polyclonal Antibody

Positive test controls include: HepG2, HeLa, 293T, RAW264.7, NIH/3T3, Mouse lung. Immunogen sequence: MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF.

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

Specifications

• Antigen: GTF3C3
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.98 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene: GTF3C3
• Gene Accession No.: Q9Y5Q9
• Gene Alias: AL022818; general transcription factor 3C polypeptide 3; general transcription factor IIIC subunit 3; general transcription factor IIIC, polypeptide 3; general transcription factor IIIC, polypeptide 3, 102kDa; GTF3C3; im:7136784; LOC553318 protein; TF3C3; TF3C-gamma; TFIIIC 102 kDa subunit; TFIIIC102; TFiiiC2-102; TFIIICgamma; Transcription factor IIIC 102 kDa subunit; Transcription factor IIIC subunit gamma; transcription factor IIIC-gamma subunit
• Gene Symbols: GTF3C3
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GTF3C3 (NP_036218.1).
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 316810, 9330, 98488
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG