H-2Dd Mouse anti-Mouse, Brilliant Violet 421, Clone: 34-2-12, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742460

Catalog No. BDB742460

Description

The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]. The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: H-2Dd
• Clone: 34-2-12
• Conjugate: Brilliant Violet 421
• Gene Alias: Histocompatibility-2Dd, H-2Dd
• Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov