H-2Dd Mouse anti-Mouse, Brilliant Violet 650, Clone: 34-2-12, BD Optibuild™

Catalog No.	Quantity
BDB742463	50 μg

Catalog No. BDB742463 Supplier BD Biosciences Supplier No. 742463

Description

Mouse Monoclonal Antibody

The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]. The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: H-2Dd
• Classification: Monoclonal
• Clone: 34-2-12
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Histocompatibility-2Dd, H-2Dd
• Host Species: Mouse
• Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov