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H-2Dd Mouse anti-Mouse, Brilliant Violet 711, Clone: 34-2-12, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742464

Catalog No. BDB742464


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Description

Description

The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]. The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
Specifications

Specifications

H-2Dd
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Histocompatibility-2Dd, H-2Dd
(C57BL/6 x DBA/2)F1 mouse splenocytes
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
34-2-12
Brilliant Violet 711
Affinity Purified
Murine
Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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