H-2Kd Mouse anti-Mouse, Brilliant Violet 786, Clone: SF1-1.1, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742436
The SF1-1.1 antibody reacts with the 3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v ) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e).The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|H-2kd, H2kd, MHC class I H-2Kd|
|BALB/c Mouse Cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 786|
|Mouse SJL IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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