H-2Ld/H-2Db Mouse anti-Mouse, Brilliant Violet 510, Clone: 28-14-8, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742469
The 28-14-8 antibody reacts with theα3 domain of the H-2D[b] MHC class I alloantigen. The antibody binds to H-2D[b] in the presence or absence of theβ2 microglobulin chain. It cross-reacts with theα3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]. Reactivity with haplotypes k, f, p, r, /and s /has not been observed. mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells.The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|H-2Db/H-2Ld, H2-Db/H2-Ld, Histocompatibility-2 Db/Ld|
|C3H.SW mouse splenocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 510|
|Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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