H-2Ld/H-2Db Mouse anti-Mouse, Brilliant Violet 650, Clone: 28-14-8, BD Optibuild™

Catalog No.	Quantity
BDB742471	50 μg

Catalog No. BDB742471 Supplier BD Biosciences Supplier No. 742471

Description

Mouse Monoclonal Antibody

The 28-14-8 antibody reacts with theα3 domain of the H-2D[b] MHC class I alloantigen. The antibody binds to H-2D[b] in the presence or absence of theβ2 microglobulin chain. It cross-reacts with theα3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]. Reactivity with haplotypes k, f, p, r, /and s /has not been observed. mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: H-2Ld/H-2Db
• Classification: Monoclonal
• Clone: 28-14-8
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: H-2Db/H-2Ld, H2-Db/H2-Ld, Histocompatibility-2 Db/Ld
• Host Species: Mouse
• Immunogen: C3H.SW mouse splenocytes
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov