Invitrogen™ HAI-1 Monoclonal Antibody (9B10), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-139-26	Invitrogen™ 14996082	100 μg

Description

Description: The monoclonal antibody 9B10 recognizes HAI-1 also known as hepatocyte growth factor activation inhibitor. HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers. Applications Reported: This 9B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) under nonreducing conditions only. Applications Tested: This 9B10 antibody has been tested by flow cytometric analysis of human cell line MCF7. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturi...

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

Specifications

• Antigen: HAI-1
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O43278
• Gene ID (Entrez): 6692
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 9B10
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human
• Gene: SPINT1
• Gene Alias: HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; hepatocyte growth factor activator inhibitor type 1; hepatocyte growth factor activator inhibitor-1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; Spint1; UNQ223/PRO256
• Gene Symbols: SPINT1
• Product Type: Antibody