CD27 Hamster anti-Mouse, Brilliant Violet 650, Clone: LG.3A10, BD Optibuild™

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 740491

Catalog No. BDB740491

Description

The LG.3A10 monoclonal antibody specifically binds to CD27, a lymphocyte-restricted member of the Tumor Necrosis Factor Receptor family which binds to CD70. The CD27 molecule is a 45-kDa transmembrane glycoprotein which is constitutively expressed by lymphocytes of the T lineage: virtually all thymocytes and over 90% of peripheral T cells bearing both αβ and γδ T-cell receptors. CD27 cooperates with the pre-TCR in mediating thymocyte differentiation and expansion. In addition, one to ten percent of mature peripheral B cells express CD27, and CD27’s role in the differentiation of human plasma cells has been studied. Mouse NK cells, freshly isolated and IL-2-activated, also express CD27. In the bone marrow, CD27 is found on a progenitor population which provides short-term hematopoietic reconstitution. Cells of the myeloid lineage do not express CD27. Cross-linked LG.3A10 mAb has been reported to amplify the proliferative response of purified T lymphocytes to suboptimal mitogenic stimulation1 and to enhance NK-cell proliferation and IFN- γproduction. In contrast, non-cross-linked LG.3A10 mAb inhibits CD3-induced pre-T cell development by interfering with the receptor-ligand interaction. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with a similar population of rat leukocytes.

Specifications

• Antigen: CD27
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Tnfrsf7; Tumor necrosis factor receptor superfamily member 7; Tp55; S152
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: LG.3A10
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only