CD28 Hamster anti-Mouse, Brilliant Violet 786, Clone: 37.51, BD Optibuild™

Catalog No.	Quantity
BDB740859	50 μg

Catalog No. BDB740859 Supplier BD Biosciences Supplier No. 740859

Description

Hamster Monoclonal Antibody

The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications

• Antigen: CD28
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 37.51
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Description: Cd28; CD28 antigen; T-cell-specific surface glycoprotein CD28
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Hamster
• Immunogen: Mouse EL-4 (T-cell lymphoma) Cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2 λ1

For Research Use Only