CD29 Hamster anti-Mouse, Brilliant Violet 605, Clone: HM β1-1, BD Optibuild™

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 740365

Catalog No. BDB740365

Description

The HM β 1-1 monoclonal antibody specifically binds to the 130-kDa integrin β 1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin- α chains. With α 1 through α 6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with α v (CD51), it forms α α 8, and α 9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes. This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes. Source of the immunogen was purified mouse VLA-4 ( α 4β 1, CD49d/CD29).

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: CD29
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2 λ1

• Applications: Flow Cytometry
• Clone: HM β1-1
• Conjugate: Brilliant Violet 605
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Purified mouse VLA-4
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only