CD49b Hamster anti-Mouse, Brilliant Violet 510, Clone: HMα2, BD Optibuild™

Catalog No.	Quantity
BDB740133	50 μg

Catalog No. BDB740133 Supplier BD Biosciences Supplier No. 740133

Description

Hamster Monoclonal Antibody

The HM α 2 antibody reacts with integrin α 2 chain (CD49b), the 150-kDa transmembrane glycoprotein that non-covalently associates with the integrin β 1 subunit (CD29) to form the integrin α 2β 1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets; but it is not on thymocytes or Peyer’s-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HM α 2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HM α

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD49b
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HMα2
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 510
• Description: Integrin α2 chain
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Hamster
• Immunogen: Mouse colon carcinoma cell line Colon26
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only