HAP1, Mouse anti-Mouse, Rat, Clone: 1B6, Millipore Sigma™

Catalog No.	Quantity
MABN2456100	100 μL
MABN245625	25 μL

Catalog No. MABN2456100 Supplier MilliporeSigma Supplier No. MABN2456100UL

Description

Mouse Monoclonal Antibody

Anti-HAP1, clone 1B6, Cat. No. MABN2456, is a highly specific mouse monoclonal antibody that targets Huntingtin-associated protein 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Huntingtin-associated protein 1 (UniProt: P54256; also known as HAP1) is encoded by the Hap1 gene (Gene ID: 29430) in rat. HAP1 is a huntingtin-associated protein that is enriched in the brain. It specifically associates with HTT/huntingtin and this binding is enhanced by an expanded polyglutamine repeats within HTT that affects HAP1 interaction properties. Both HTT and HAP1 are reported to be involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. HAP1 localizes to large nonmembrane-bound cytoplasmic bodies (stigmoid bodies) in various types of neurons. The formation of stigmoid bodies is regulated by the ratio of isoform A to isoform B of HAP1. HAP1 plays a role in vesicular transport within neurons that involves microtubule-associated transporters and is shown to be attenuated by association with mutant HTT. In rodents, two HAP1 isoforms (HAP1A and HAP1B) have been described that differ in their carboxy terminal. However, humans contain only the HAP1A isoform. In murine models, targeted disruption of the HAP1 gene results in significantly depressed feeding behavior and premature postnatal death. Pups with Hap1 -/- show decreased feeding, malnutrition, dehydration, and premature death. (Ref.: Tang, TS et al., (2003). Neuron 39 (2); 227-239; Chan, EY., et al. (2002). Hum. Mol. Gen. 11(8); 945-959).

Specifications

• Antigen: HAP1
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 1B6
• Conjugate: Unconjugated
• Dilution: Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Huntingtin-associated protein 1
• Gene Symbols: Hap1
• Host Species: Mouse
• Immunogen: GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
• Purification Method: Protein G purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_077047.1
• Test Specificity: Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
• Target Species: Mouse, Rat
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ