Invitrogen™ HAS3 Monoclonal Antibody (3C9)

Catalog No.	Quantity
PIMA517088	100 μg

Catalog No. PIMA517088 Supplier Invitrogen™ Supplier No. MA517088

Description

Mouse Monoclonal Antibody

MA5-17088 targets HAS3 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17088 immunogen is purified recombinant fragment of human HAS3 expressed in E. Coli. MA5-17088 detects HAS3 which has a predicted molecular weight of approximately 63kDa.

HAS1, HAS2 and HAS3 are HA (hyaluronan or hyaluronic acid) synthase proteins. The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesize high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults. HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis. Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGFbeta downregulates HAS3 transcript levels. The human HAS3 gene maps to chromosome 16q22. 1.

Specifications

• Antigen: HAS3
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 3C9
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: HAS3
• Gene Accession No.: O00219
• Gene Alias: HA synthase 3; Has3; Hyaluronan synthase 3; hyaluronate synthase 3; Hyaluronic acid synthase 3
• Gene Symbols: HAS3
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human HAS3 expressed in E. Coli.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3038
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1