Invitrogen™ HBEGF Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P32950UG

Catalog No. 500P32950UG

Description

AA Sequence of recombinant protein: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography employing an immobilized Human HB-EGF matrix. Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Rabbit Anti-Human HB-EGF (500-P329BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human HB-EGF. Western Blot: To detect Human HB-EGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

HBEGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function. HBEGF promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. It also acts as a diphtheria toxin receptor.

Specifications

• Antigen: HBEGF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HBEGF
• Gene Alias: AW047313; Diphtheria toxin receptor; Diphtheria toxin receptor (heparin binding epidermal growth factor - like growth factor); diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); DTR; DT-R; DTS; DTSF; GFHB; hb egf; HBEGF; Hb-egf; Hegfl; heparin binding EGF like growth factor; heparin binding epidermal growth factor-like growth factor; heparin-binding EGF-like growth factor; heparin-binding epidermal -growth - like growth factor; heparin-binding epidermal growth factor; heparin-binding epidermal -growth factor; proheparin-binding EGF-like growth factor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1839
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q99075
• Gene Symbols: HBEGF
• Immunogen: E.coli-derived, 9.7 kDa Recombinant Human HB-EGF.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody