Invitrogen™ HCMV IL-10 Polyclonal Antibody

Catalog No.	Quantity
PIPA547082	100 μg

Catalog No. PIPA547082 Supplier Invitrogen™ Supplier No. PA547082

Description

Goat Polyclonal Antibody

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-10, recombinant canine IL-10, recombinant feline IL-10, recombinant cotton rat IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant human IL-10, and recombinant Epstein-Barr virus encoded viral IL-10 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 μg of the antibody by the LAL method.

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses. It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection. Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus' advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection.

Specifications

• Antigen: HCMV IL-10
• Applications: Neutralization, Western Blot
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Alias: CSIF; CSIFMGC126450; IL10A; interleukin 10; interleukin-10; TGIF
• Host Species: Goat
• Immunogen: E. coli-derived recombinant viral HCMV IL-10 Ala26-Lys176.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Virus
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

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