Invitrogen™ HCN1 Monoclonal Antibody (S70)

Catalog No.	Quantity
PIMA527589	100 μg

Catalog No. PIMA527589

Supplier: Invitrogen™ MA527589

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-27589 was sufficient for detection of HCN1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 100kDa. No cross-reactivity against HCN2.

HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.

Specifications

• Antigen: HCN1
• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Microarray
• Classification: Monoclonal
• Clone: S70
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene: HCN1
• Gene Accession No.: O60741, O88704, Q9JKB0
• Gene Alias: Bcng1; BCNG-1; Brain cyclic nucleotide-gated channel 1; C630013B14Rik; EIEE24; Hac2; HAC-2; Hcn1; hyperpolarization activated cyclic nucleotide gated potassium channel 1; hyperpolarization-activated cation channel 2; hyperpolarization-activated cyclic nucleotide-gated potassium channel 1; hyperpolarization-activated, cyclic nucleotide-gated K+ 1; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• Gene Symbols: HCN1
• Host Species: Mouse
• Immunogen: Fusion protein amino acids 778-910 (C terminus) of rat HCN1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15165, 348980, 84390
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1