Invitrogen™ HCoV-229E Spike S2 Polyclonal Antibody

Catalog No.	Quantity
PIPA5120721	100 μL

Catalog No. PIPA5120721 Supplier Invitrogen™ Supplier No. PA5120721

Description

Rabbit Polyclonal Antibody

Positive test controls include: Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag). Immunogen sequence: LENKSAELNY TVQKLQTLID NINSTLVDLK WLNRVETYIK WPWWVWLCIS VVLIFVVSML LLCCCSTGCC GFFSCFASSI RGCCESTKLP YYDVEKIHIQ.

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [Uniprot].

Specifications

• Antigen: HCoV-229E Spike S2
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 1.75 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Alias: 229E; Coronavirus; HCoV; S2; Spike
• Host Species: Rabbit
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of coronavirus Spike S2.
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Virus
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG