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Invitrogen™ HCS LIVE/DEAD™ Green Kit

Catalog No. H10290
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Catalog No. H10290 Supplier Invitrogen™ Supplier No. H10290
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Description

The HCS LIVE/DEAD™ Green Kit measures cytotoxicity by high content analysis with the Image-iT™ DEAD Green™ viability stain—a non-fluorescent and cell impermeant compound that exhibits a strong fluorescent enhancement upon binding to DNA.

The HCS LIVE/DEAD™ Green Kit measures cytotoxicity by high content analysis with the Image-iT™ DEAD Green™ viability stain—a non-fluorescent and cell impermeant compound that exhibits a strong fluorescent enhancement upon binding to DNA. Drugs and test compounds leading to serious cell injuries, including plasma membrane permeability, allow entry of the Image-iT™ DEAD Green™ viability stain. This two-color fluorescence assay also employs the use of a cell permeant nuclear segmentation tool that stains both live and dead cells, reflecting the total cell number within the sample. For total cell staining, the kit includes both a blue-fluorescent Hoechst 33342 and a near infrared-fluorescent, HCS NuclearMask™ Deep Red reagent. Either of these segmentation tools may be used with Image-iT™ DEAD Green™ viability stain for excellent discrimination of live and dead cells that survives fixation and permeabilization for combination with additional parameters of cytotoxicity or other biomarkers. The kit contains sufficient reagents for performing 2, 96-well plates.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Eukaryotic Cells
Color Green, Far-red, Blue
Content And Storage Store at ≤-20°C, desiccate and protect from light.
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, High Content Analysis Instrument
Product Type HCS LIVE/DEAD Green Kit
Dye Type HCS NuclearMask™ Deep Red stain, Image-iT™ DEAD Green viability stain, Hoechst 33342
Format 96-well plate
Product Line LIVE/DEAD, Molecular Probes
Quantity 1 kit
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

For Research Use Only. Not for use in diagnostic procedures.

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