Invitrogen™ HDAC1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532443

Catalog No. PIPA532443

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or breast carcinoma.

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

Specifications

• Antigen: HDAC1
• Classification: Polyclonal
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: O09106, Q13547
• Gene Symbols: HDAC1
• Immunogen: Synthetic peptide derived from C-terminus of human HDAC-1 protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: HDAC1
• Gene Alias: DKFZp686H12203; GON-10; HD1; Hdac1; Hdac1-ps; Histone deacetylase 1; histone deacetylase 1-like protein; MommeD5; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2; RPD3; RPD3L1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3065, 433759
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid