HDAC8 (near N-terminus)Rabbit anti-Human, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-2260	100 μg

Catalog No. 50-199-2260 Supplier Rockland Immunochemicals Supplier No. 600401J72

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. A BLAST analysis was used to suggest cross-reactivity with rat, mouse, human, and bovine based on 100% sequence homology. Cross-reactivity with HDAC8 from other sources has not been determined.

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Specifications

• Antigen: HDAC8 (near N-terminus)
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 30% glycerol and no preservative; pH 7.2
• Gene: HDAC8
• Gene Accession No.: Q9BY41
• Gene Alias: 2610007D20Rik; CDA07; CDLS5; HD8; HDAC8; HDACL1; Histone deacetylase 8; histone deacetylase-like 1; MRXS6; RGD1562895; RPD3; WTS
• Gene Symbols: HDAC8
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 55869
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid