Invitrogen™ HDAC8 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA579353

Catalog No. PIPA579353

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Rat Cardiac Muscle: Tissue, A549 whole cell. IHC: Human Lung Cancer Tissue.

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Specifications

• Antigen: HDAC8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HDAC8
• Gene Alias: 2610007D20Rik; CDA07; CDLS5; HD8; Hdac8; HDACL1; histone deacetylase 8; histone deacetylase-like 1; MRXS6; RGD1562895; RPD3; WTS
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 363481, 55869, 70315
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: B1WC68, Q8VH37, Q9BY41
• Gene Symbols: HDAC8
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC8 (18-33aa YIYSPEYVSMCDSLAK).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG