Invitrogen™ HDEL Monoclonal Antibody (2E7), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545240

Catalog No. PIMA545240

Description

1 μg/mL of MA5-45240 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78kDa.

HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.

Specifications

• Antigen: HDEL
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: O76767
• Gene Symbols: KdelR
• Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Drosophila, Yeast, Plant
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Western Blot, Immunocytochemistry
• Clone: 2E7
• Conjugate: APC
• Gene: KdelR
• Gene Alias: BEST:CK00230; CG5183; CG5183-PA; CG5183-PB; CG5183-PC; CK00230; Dmel\CG5183; Dmel_CG5183; dmErd2; ER lumem protein retaining receptor 2; ER lumen protein retaining receptor; ER lumen protein retaining receptor 2; ER lumen protein-retaining receptor; Erd2; KDEL receptor; KdelR; KDELR1; KDEL-receptor; KdelR-PA; KdelR-PB; KdelR-PC; l(2)31Em; l(2)k00311; lethal (2) 31Em
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 34427
• Content And Storage: 4°C
• Form: Liquid