Helios Hamster, Alexa Fluor 488, Clone: 22F6, BD
Armenian Hamster Monoclonal Antibody
Manufacturer: BD Biosciences 563950
The 22F6 monoclonal antibody specifically binds to mouse and human Helios. Helios is a member of the Ikaros family of zinc-finger transcription factors, which play important roles in hematopoietic cell development and tumor suppression. Helios expression is restricted to the earliest stages of embryonic hematopoiesis, a variety of epithelial tissues and is notably increased in thymic-derived regulatory CD4+Foxp3+ T (Treg) cells. Its high expression levels in Treg cells are independent from Foxp3 and are believed to contribute, along with other transcription factors, to the phenotypic stability of natural regulatory T cells. Accordingly, it has been demonstrated that Helios directly stimulates Foxp3 transcription while it inhibits ll2 /gene expression, contributing for the maintenance of cellular anergy. Helios is also differentially expressed during negative and positive selection in the thymus, marking CD4+ autoreactive cells for deletion. Helios may possibly play roles in T cell activation, since it is upregulated in Th2 and Tfh cells. Despite these roles in T cell development and function, Helios genetic ablation in mice revealed no significant abnormalities in Treg or other T cell subsets. This finding suggests that other Ikaros family members may play redundant roles.Alexa Fluor™ conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of FITC. However, Alexa Fluor™ 488 tends to be brighter and more optimal for intracellular applications. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor™ 488 cannot be used simultaneously. Alexa Fluor™ 488 exhibits extraordinary photostability, which makes it highly suitable for fluorescence microscopy.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IKAROS family zinc finger protein 2, ANF1A2, HELIOS, ZNF1A2, ZNFN1A2|
|Mouse Helios Peptide|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 488 under optimum conditions, and unreacted Alexa Fluor™ 488 was removed.|
|Alexa Fluor 488|
|Armenian Hamster IgG|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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