Helios Hamster, Alexa Fluor 488, Clone: 22F6, BD

Armenian Hamster Monoclonal Antibody

Supplier:  BD Biosciences 563950

Catalog No. BDB563950

Description

The 22F6 monoclonal antibody specifically binds to mouse and human Helios. Helios is a member of the Ikaros family of zinc-finger transcription factors, which play important roles in hematopoietic cell development and tumor suppression. Helios expression is restricted to the earliest stages of embryonic hematopoiesis, a variety of epithelial tissues and is notably increased in thymic-derived regulatory CD4+Foxp3+ T (Treg) cells. Its high expression levels in Treg cells are independent from Foxp3 and are believed to contribute, along with other transcription factors, to the phenotypic stability of natural regulatory T cells. Accordingly, it has been demonstrated that Helios directly stimulates Foxp3 transcription while it inhibits ll2 /gene expression, contributing for the maintenance of cellular anergy. Helios is also differentially expressed during negative and positive selection in the thymus, marking CD4+ autoreactive cells for deletion. Helios may possibly play roles in T cell activation, since it is upregulated in Th2 and Tfh cells. Despite these roles in T cell development and function, Helios genetic ablation in mice revealed no significant abnormalities in Treg or other T cell subsets. This finding suggests that other Ikaros family members may play redundant roles.

Alexa Fluor™ conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of FITC. However, Alexa Fluor™ 488 tends to be brighter and more optimal for intracellular applications. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor™ 488 cannot be used simultaneously. Alexa Fluor™ 488 exhibits extraordinary photostability, which makes it highly suitable for fluorescence microscopy.

Specifications

• Antigen: Helios
• Clone: 22F6
• Conjugate: Alexa Fluor 488
• Gene Alias: IKAROS family zinc finger protein 2, ANF1A2, HELIOS, ZNF1A2, ZNFN1A2
• Immunogen: Mouse Helios Peptide
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Armenian Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG