Invitrogen™ HELIOS Monoclonal Antibody (22F6), PE-Cyanine5, eBioscience™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
15-988-342	Invitrogen™ 15988342	100 Tests

Description

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia. 22F6 has been reported to cross react with rat Helios. Applications Reported: This 22F6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 22F6 antibody has been pre-diluted and tested by flow cytometric analysis of mouse lymph node cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at www.thermofisher.com/flowprotocols . This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range ...

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Specifications

• Antigen: HELIOS
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine5
• Host Species: Armenian Hamster
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P81183, Q9UKS7
• Gene ID (Entrez): 22779, 22807, 488510
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 22F6
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human, Mouse, Canine
• Gene: IKZF2
• Gene Alias: A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2
• Gene Symbols: IKZF2
• Product Type: Antibody