Hematopoietic Lineage Rat anti-Mouse, eFluor® 450, eBioscience
Manufacturer: Invitrogen 88777272
Description: The Mouse Hematopoietic Lineage eFluor 450 Cocktails contains eFluor 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens. This cocktail contains the following antibodies: Anti-Mouse CD3 (17A2) eFluor 450 Anti-Mouse CD45R (B220) (RA3-6B2) eFluor 450 Anti-Mouse CD11b (M1/70) eFluor 450 Anti-Mouse TER-119 (TER-119) eFluor 450 Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) eFluor 450 Applications Reported: The Mouse Hematopoietic Lineage eFluor 450 Cocktail has been reported for use in flow cytometric analysis. Applications Tested: The Mouse Hematopoietic Lineage eFluor 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
|Mouse Hematopoietic Lineage|
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Hematopoietic Lineage Cocktail|
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