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Precipio™ HemeScreen AML Panel-1

Catalog No. 22021970
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22-021-970 1 Sample
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Catalog No. 22-021-970 Supplier Precipio™ Supplier No. HS1PAML
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Description

The HemeScreen AML Panel-1 is an RUO pre-plated kit used to detect AML-related gene mutations for IDH1, IDH2, KIT, FTL3, NPM1, and CEBPA genes through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Pre-plated configuration with embedded mutant, wild type, and NTC controls to run 1 sample in duplicate
  • Simplified workflow and reduced chance of error
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Content And Storage 96-well Plate with Gene Targets, Positive, Negative, and Wild Type Controls. Store at -20°C
Control Template NTC controls
Detection Method HRM
Form Liquid
Format Kit, 96-well Plate
PCR Method qPCR
Technique HRM, qPCR
Disease Acute Myeloid Leukemia
For Use With (Equipment) PCR Instruments
Gene Symbol FLT3, KIT, IDH1, IDH2, CEBPA, NPM1
Genotyping Target Somatic Mutations
Includes Label or Dye Yes
Kit Type HRM DNA Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 1 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Diagnostic Kit
Quantity 1 Sample
Research Category Cancer
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 1 Assay
Target IDH1, IDH2, KIT, FTL3, NPM1, CEBPA
Target Loci FLT3, KIT, IDH1, IDH2, CEBPA, NPM1
Target Organism Class Human
Target Specificity >99%
For Use With (Application) Pathogen Detection
Compatibility HRM-enabled RT-PCR Instruments
No. of Reactions 1 Reaction
Reaction Format Master Mix
Reagent Type Mutation Screening
Sample Type Whole Blood, Bone Marrow
Starting Material DNA
Includes 96-well plate with gene targets, positive controls, negative controls, wild type controls, primers, mastermix
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