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Precipio™ HemeScreen BCR/ABL1 Panel-20

Catalog No. 22021973
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20 Samples
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Catalog No. Quantity
22-021-973 20 Samples
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Catalog No. 22-021-973 Supplier Precipio™ Supplier No. HS20RBCR
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Description

The HemeScreen BCR/ABL1 Panel-20 is an RUO reagent set kit used to screen for 3 major BCR/ABL1 isoforms (p190, p210, and p230) and 1 minor isoform (p203) associated with BCR/ABL1-induced leukemogenesis, including CML and ALL using HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Isoforms tested: p190, p203, p210, p230
  • Simplified workflow and reduced chance of error
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Calibration Type HRM Calibration
Content And Storage 3 x 5 mL Tube Primer Sets, 4 x 1.5 mL Tube Mutant Controls. Store at -20°C
Detection Method HRM
Form Liquid
Format Kit, Tube(s)
PCR Method RT-qPCR
Technique HRM
Disease Chronic Myeloid Leukemia and Acute Lymphocytic Leukemia
For Use With (Equipment) PCR Instruments
Genotyping Target BCR/ABL1 Fusion Transcripts
Includes Label or Dye Yes
Kit Type BCR ABL1 Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 20 Test
Primer PCR Primers
Product Line HemeScreen
Product Type PCR Assay Kit (HRM)
Quantity 20 Samples
Research Category Cancer, Hematology
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 20 Samples
Target BCR/ABL1
Target Organism Class Human
Target Specificity >99%
For Use With (Application) Genetic Disease Research
Compatibility HRM-enabled RT-PCR Instruments
Final Product Type BCR/ABL1 Transcripts
No. of Reactions 20 Reactions
Reaction Format Master Mix
Reagent Type PCR Reagent Kit
Sample Type RNA
Starting Material RNA
Includes 3 x 5 mL Primer sets, 4 x 1.5 mL Mutant controls
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