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Precipio™ HemeScreen DNMT3A Kit-100

Catalog No. 22021986
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100 Samples
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Catalog No. Quantity
22-021-986 100 Samples
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Catalog No. 22-021-986 Supplier Precipio™ Supplier No. HSFFDNMT3A
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Description

The HemeScreen DNMT3A Kit-100 is an RUO reagent set kit used to detect DNMT3A gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Content And Storage 5 mL Tube Primer Set, 1.5 mL Tube Mutant Control. Store at -20°C
Control Template Mutant Control
Detection Method HRM
Form Liquid
Format Kit, Pre-plated
PCR Method qPCR
Technique RT-PCR, HRM
For Use With (Equipment) PCR Instruments
Gene Symbol DNMT3A
Genotyping Target SNPs, Indels, Modifications
Includes Label or Dye Yes
Kit Type HRM DNMT3A Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Mutation Detection Kit (HRM)
Quantity 100 Samples
Research Category Genetics, Cancer Research
Sensitivity 0.95
Shipping Condition Dry Ice
Software QuantStudio™ v1.5.1 software; High Resolution Melt Software v3.2
Sufficient For 100 Samples
Target DNMT3A
Target Loci DNMT3A (exon 23)
Target Organism Class Human
Target Specificity >99%
For Use With (Application) Genotyping
Activation Time 3 min.
Compatibility Most RT-PCR Instruments, HRM-enabled RT-PCR Instruments
Final Product Type Mutated DNA
No. of Reactions 100 Reactions
Reaction Format Master Mix
Reagent Type Mutation Detection Reagent
Sample Type Peripheral Blood, Bone Marrow
Includes 5 mL tube primer set, 1.5 mL tube mutant control
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