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Precipio™ HemeScreen FLT3 Kit-100

Catalog No. 22021981
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100 Samples
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Catalog No. Quantity
22-021-981 100 Samples
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Catalog No. 22-021-981 Supplier Precipio™ Supplier No. HSFFFLT3
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Description

The HemeScreen FLT3 Kit-100 is an RUO reagent set kit used to detect FLT gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Concentration 1X
Content And Storage 4 x 5 mL Tube Primer Sets, 1 mL Tube Mutant Control. Store at -20°C
Control Template Mutant Control
Detection Method HRM
Form Liquid
Format Kit, Tube(s)
PCR Method qPCR
Technique PCR, HRM
Disease Haematological Cancers, Myeloproliferative Disorders
For Use With (Equipment) PCR Instruments
Gene Symbol FLT3
Genotyping Target FLT3 Gene Mutations
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye Yes
Kit Type HRM Gene Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Genotyping Kit (HRM)
Quantity 100 Samples
Research Category Cancer, Hematology
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 100 Samples
Target FLT
Target Loci FLT3 (Exon 14, Exon 15, Exon 16, Exon 20)
Target Organism Class Human
Target Specificity >99%
Activation Time 3 min.
Compatibility HRM-enabled RT- PCR Instruments, RT- PCR System
Reaction Format Master Mix
Reagent Type PCR Reagent Kit
Sample Type Genomic DNA
Starting Material DNA
Volume 20 μL
Includes 4 x 5 mL tube primer sets, 1 mL tube mutant control
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