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Precipio™ HemeScreen KIT Kit-100

Catalog No. 22021982
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100 Samples
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Catalog No. Quantity
22-021-982 100 Samples
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Catalog No. 22-021-982 Supplier Precipio™ Supplier No. HSFFKIT
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Description

The HemeScreen KIT Kit-100 is an RUO reagent set kit used to detect KIT gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Content And Storage 3 x 5 mL Tube Primer Sets, 1 mL Tube Mutant Control. Store at -20°C
Control Template Mutant Positive Controls
Detection Method HRM
Form Liquid
Format Kit, Tube(s)
PCR Method qPCR
Technique PCR, HRM
Disease Acute Myeloid Leukemia
For Use With (Equipment) PCR Instruments
Gene Symbol KIT
Genotyping Target KIT gene mutations
Includes Label or Dye Yes
Kit Type KIT Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Mutation Screening Kit (HRM)
Quantity 100 Samples
Research Category Cancer, Myeloproliferative Disorders
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 100 Samples
Target KIT
Target Loci KIT
Target Organism Class Human
Target Specificity >99%
Compatibility HRM-enabled RT-PCR
Final Product Type Mutated DNA
No. of Reactions 100 Reactions
Reaction Format Master Mix
Reagent Type Mutation Detection
Sample Type Whole blood, Bone marrow
Starting Material DNA
Includes 3 x 5 mL tubes primer sets, 1 mL tube mutant control
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