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Precipio™ HemeScreen MPL Exon 10 Kit-100

Catalog No. 22021977
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100 Samples
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Catalog No. Quantity
22-021-977 100 Samples
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Catalog No. 22-021-977 Supplier Precipio™ Supplier No. HSFFMPL
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Description

The HemeScreen MPL Exon 10 Kit-100 is an RUO reagent set kit used to detect MPL Exon 10 gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Calibration Type HRM Calibration
Content And Storage 5 mL Tube Primer Set, 1.5 mL Tube Mutant Control. Store at -20°C
Control Template Mutant Control
Detection Method HRM
Form Liquid
Format Kit, Tube(s)
PCR Method qPCR
Technique PCR, HRM Analysis
For Use With (Equipment) PCR Instruments
Gene Symbol MPL
Genotyping Target MPL Exon 10 Mutations
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye Yes
Kit Type HRM PCR DNA Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Optimal Reaction Temperature 68°C
Primer PCR Primers
Product Line HemeScreen
Product Type Genotyping Kit
Quantity 100 Samples
Research Category Genetics, Oncology
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 100 Samples
Target MPL
Target Loci MPL (Exon 10)
Target Organism Class Human
Target Specificity >99%
Activation Time 3 min.
Compatibility HRM-enabled RT-PCR Instruments
Final Product Type Genomic DNA
No. of Reactions 100 Reactions
Reaction Format Master Mix
Reagent Type Genotyping, High Resolution Melt
Sample Type DNA, Whole Blood, Bone Marrow
Starting Material DNA
Includes 5 mL tube primer set, 1.5 mL tube mutant control
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