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Precipio™ HemeScreen NOTCH1 Kit-100

Catalog No. 22021991
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100 Samples
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Catalog No. Quantity
22-021-991 100 Samples
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Catalog No. 22-021-991 Supplier Precipio™ Supplier No. HSFFNOTCH1
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Description

The HemeScreen NOTCH1 Kit-100 is an RUO reagent set kit used to detect NOTCH1 gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Content And Storage 5 mL Tube Primer Set, 1.5 mL Tube Mutant Control. Store at -20°C
Detection Method HRM
Form Liquid
Format Kit, Tube(s)
PCR Method qPCR
Technique PCR, HRM Analysis
Disease Chronic Lymphocytic Leukemia
For Use With (Equipment) PCR Instruments
Gene Symbol NOTCH1
Genotyping Target NOTCH1 Mutations
Includes Label or Dye Yes
Kit Type HRM DNA Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Mutation Detection Kit
Quantity 100 Samples
Research Category Cancer Research, Hematology
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 100 Samples
Target NOTCH1
Target Loci NOTCH1 (Exon 34)
Target Organism Class Human
Target Specificity >99%
Compatibility HRM-enabled RT-PCR Instruments
No. of Reactions 100 Reactions
Reaction Format Master Mix
Reagent Type Mutation Screening, PCR Reagents
Sample Type Whole Blood, Bone Marrow
Starting Material DNA
Includes 5 mL tube primer set, 1.5 mL tube mutant control
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