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Precipio™ HemeScreen SF3B1 Kit-100

Catalog No. 22021989
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100 Samples
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Catalog No. Quantity
22-021-989 100 Samples
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Catalog No. 22-021-989 Supplier Precipio™ Supplier No. HSFFSF3B1
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Description

The HemeScreen SF3B1 Kit-100 is an RUO reagent set kit used to detect SF3B1 gene mutations through HRM methods.

HemeScreen is a proprietary set of RUO (Research Use Only) reagents used to screen the wild type (Negative) from Mutated (Positive) genes in a simplified workflow relative to alternative molecular testing technologies (RT-PCR or NGS).

  • Simplified workflow and reduced chance of error
  • Contains primers mixed with master mix to run 100 samples in duplicate, and mutant controls to run 5 batches
  • Data capture during thermocycling
  • Fast turnaround time
  • Broad mutation detection (SNPs, Modifications, Indels)
  • Low limits of detection
  • Results are qualitative and DNA sequencing is required to identify specific isoforms
  • LOD: 5%
  • Assay includes Primers-Mastermix Mix and Positive controls
  • Requires HRM-enabled RT-PCR (example Thermo Fisher™ Quantstudio 3 or higher)

Specifications

Content And Storage 3 x 5 mL Tube Primer Sets, 1 mL Tube Mutant Control. Store at -20°C
Control Template Mutant Positive Controls
Detection Method HRM
Form Liquid
Format Kit, 96-well plate
PCR Method qPCR
Technique HRM
For Use With (Equipment) PCR Instruments
Gene Symbol SF3B1
Genotyping Target SF3B1 Gene Mutations
High-throughput Compatibility High-throughput Compatible
Includes Label or Dye Yes
Kit Type HRM Gene Mutation Detection Kit
Label or Dye High Resolution Melting (HRM)
No. of Tests 100 Test
Primer PCR Primers
Product Line HemeScreen
Product Type Genotyping Kit (HRM)
Quantity 100 Samples
Research Category Genetics, Hematology
Sensitivity 0.95
Shipping Condition Dry Ice
Sufficient For 100 Samples
Target SF3B1
Target Loci SF3B1
Target Organism Class Human
Target Specificity >99%
Compatibility HRM-enabled RT-PCR Instruments, RT- PCR System
Reaction Format Master Mix
Reagent Type Mutation Detection
Sample Type Peripheral Blood, Bone Marrow
Starting Material DNA
Includes 3 x 5 mL tube primer sets, 1 mL tube mutant control
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