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Invitrogen™ Hemoglobin Chimeric Recombinant Rabbit Monoclonal Antibody (2A1)
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Catalog No. PIMA548118
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200 μg
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PIMA548118 200 μg
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Catalog No. PIMA548118 Supplier Invitrogen™ Supplier No. MA548118
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Description

Rabbit Recombinant Monoclonal Antibody

Specificity: 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb. Preparation: Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruends Adjuvant.

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Hemoglobin Chimeric
Applications ELISA, Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone 2A1
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% ProClin 300
Gene HBB
Gene Accession No. P01942
Gene Alias alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; delta globin; Haemoglobin; Hb; HBA1; HbA1c; HBA2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin subunit alpha 1; hemoglobin, alpha 1
Gene Symbols HBA, HBB
Host Species Rabbit
Immunogen Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals.
Purification Method Protein A
Quantity 200 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 15121, 15127
Target Species Mouse
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG κ
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