Heparanase-1, Rabbit anti-Human, Mouse, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABC1687100UG

Catalog No. ABC1687100

Description

Anti-Heparanase-1, Cat. No. ABC1687, is a rabbit polyclonal antibody that detects Heparanase-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Heparanase (UniProt: Q9Y251; also known as EC: 3.2.1.166, Endo-glucuronidase, Heparanase-1, Hpa1) is encoded by the HPSE (also known as HEP, HPA, HPA1, HPR1, HPSE1, HSE1) gene (Gene ID: 10855) in human. Heparanase is a heterodimeric, glycosylated, endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans and participates in extracellular matrix (ECM) degradation and remodeling. It is known to selectively cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. It is also shown to cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. Heparanase is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver, and tumor tissues. Heparanase is synthesized with a signal peptide (aa 1-35) and a propeptide chain (110-157), which are cleaved off to produce the heterodimeric mature form of 8 kDa and 50 kDa subunits. This active 8/50 kDa heterodimer is resistant to degradation. Heparanase is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Heparanase activity is inhibited by EDTA and laminarian sulfate. Four isoforms of heparanase have been described that are produced by alternative splicing.

Specifications

• Antigen: Heparanase-1
• Classification: Polyclonal
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Heparanase-1 in human spleen and human placenta tissue sections.Western Blotting Analysis: 1μg/mL from a representative lot detected Heparanase-1 in human spleen tissue lysate.
• Gene Alias: Endo-glucoronidase;Heparanase;Hpa1
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽59 kDa observed; 61.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 100 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_001092010
• Target Species: Human, Mouse

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: HPSE;HEP;HPA;HPA1;HPR1;HPSE1;HSE1
• Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of larger subunit (50 kDa)of human Heparanase-1.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects human Heparanase-1. It targets an epitope with in 13 amino acids from the C-terminal half of larger subunit (50 kDa).
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.