Hexokinase Polyclonal Antibody, Biotin, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 2004659S

Catalog No. 50-199-1181

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other sources may occur but have not been specifically determined.

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Specifications

• Antigen: Hexokinase
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: HXK1
• Gene Alias: hexokinase 1; Hexokinase PI; Hexokinase-1; Hexokinase-A; HK1-ta; HK1-tb; HK1-tc; HKA; HKI; HXK1; YFR053C
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Yeast
• Product Type: Antibody

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P04806
• Gene Symbols: HXK1
• Immunogen: Hexokinase [Yeast]
• Regulatory Status: RUO
• Gene ID (Entrez): 850614
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized