Invitrogen™ HIC1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA589523

Catalog No. PIPA589523

Description

Immunogen sequence: AATPVIQACY PSPVGPPPPP AAEPPSGPEA AVNTHCAELY ASGPGPAAAL CASERRCSPL CGLDLSKKSP PGSAAPERPL AERELPPRPD SPPSAGPAAY KEPPLALPSL PPLPFQKLEE AAPPSDPFRG GSGSPGPEPP GRPDGPSLLY RWMKHEPGLG SYGDELGRER GSPSERCEER GGDAAVSPGG PPLGLAPPPR YPGSLDGPGA GGDGDDYKSS SEETGSSEDP SPPGGHLEGY PCPHLAYGEP E; Positive Samples: Mouse liver; Cellular Location: Nucleus.

Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and the expression of HIC-1 is also frequently suppressed in leukemia and various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad complex POZ (or BTB) domain. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.

Specifications

• Antigen: HIC1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Hic1
• Gene Alias: AA408311; HIC1; hic-1; hypermethylated in cancer 1; hypermethylated in cancer 1 protein; ZBTB29; Zinc finger and BTB domain-containing protein 29; ZNF901
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15248
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.6 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q9R1Y5
• Gene Symbols: Hic1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_0010916721).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG