HIF-1 alpha Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NB100449

Catalog No. NB100449

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: TBS, 0.1% BSA with 0.09% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Host Species: Rabbit
• Molecular Weight of Antigen: 93 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown
• Concentration: 0.2 mg/mL
• Dilution: Western Blot 1:2000 - 1:10000, Simple Western 1:200, Flow Cytometry 0.125 ug per 1 million cells in a 150 mcl reaction, ELISA 1:100-1:2000, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 2-5 ug/mg lysate, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated, Knockdown Validated
• Gene Accession No.: Q16665
• Gene Symbols: HIF1A
• Immunogen: The immunogen recognized by this HIF-1 alpha Antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 [Uniprot# Q16665].
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Monkey (COS-7) and Rat (review feedback). Has been shown to detect HIF-1 alpha in both mouse and human tissue by IHC with Citrate buffer antigen retrieval. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus and Gansu zokor.
• Content And Storage: Store at 4C. Do not freeze.

For Research Use Only