HIF-1 alpha Antibody, Novus Biologicals™

Catalog No.	Quantity
NB100449	0.1 mL

Catalog No. NB100449 Supplier Novus Biologicals Supplier No. NB100449

Description

Rabbit Polyclonal Antibody

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1:2000 - 1:10000, Simple Western 1:200, Flow Cytometry 0.125 ug per 1 million cells in a 150 mcl reaction, ELISA 1:100-1:2000, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 2-5 ug/mg lysate, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated, Knockdown Validated
• Formulation: TBS, 0.1% BSA with 0.09% Sodium Azide
• Gene Accession No.: Q16665
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Host Species: Rabbit
• Immunogen: The immunogen recognized by this HIF-1 alpha Antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 [Uniprot# Q16665].
• Molecular Weight of Antigen: 93 kDa
• Purification Method: Affinity Purified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: Monkey (COS-7) and Rat (review feedback). Has been shown to detect HIF-1 alpha in both mouse and human tissue by IHC with Citrate buffer antigen retrieval. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus and Gansu zokor.
• Target Species: Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey
• Content And Storage: Store at 4C. Do not freeze.
• Isotype: IgG

For Research Use Only